Immunocytochemistry/Immunofluorescence analysis of NDUFS4 in NIH-3T3 cells using NDUFS4 Recombinant Monoclonal Antibody (Product # MA5-38178) at a dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NDUFS4 in C6 cells. Samples were incubated with NDUFS4 Monoclonal antibody (Product # MA5-38178) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Western blot analysis of NDUFS4 in extracts of various cell lines. Samples were incubated with NDUFS4 Monoclonal antibody (Product # MA5-38178) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.