Immunohistochemistry (Paraffin) analysis of COPS8 in paraffin-embedded human liver carcinoma tissue. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with COPS8 polyclonal Antibody (Product # PA5-118400) at a dilution of 1:400 for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Western Blot analysis of COPS8 using COPS8 polyclonal Antibody (Product # PA5-118400) at a dilution of 1:500 was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Lane 1: SHSY5Y cell lysate, Lane 2: HL-60 cell lysate.