Western blot analysis of CEND using CEND Recombinant Monoclonal Antibody (Product # MA5-38146) at a 1:1,000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Western blot analysis of CEND in extracts of various cell lines. Samples were incubated with CEND Monoclonal antibody (Product # MA5-38146) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.