Western blot analysis of ATBF1 on ATBF1 recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500181) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: ATBF1 recombinant protein with primary antibody at 1:1,000 dilutions Lane 2: ATBF1 recombinant protein with primary antibody at 1:5,000 dilutions