Western blot analysis of Uroplakin III on rat bladder tissue lysates with Rabbit anti-Uroplakin III antibody (HA500212) at 1/1,000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 31 kDa Observed band size: 44 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500212) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.