Western blot analysis of Hexokinase II on different lysates with Rabbit anti-Hexokinase II antibody (HA500186) at 1/500 dilution. Lane 1: 293 cell lysate (10 µg/Lane) Lane 2: Jurkat cell lysate (10 µg/Lane) Lane 3: PC-12 cell lysate (10 µg/Lane) Lane 4: NIH/3T3 cell lysate (10 µg/Lane) Lane 5: Rat skeletal muscle tissue lysate (20 µg/Lane) Predicted band size: 102 kDa Observed band size: 102 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500186) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.