MHC Class I Antibody (R1-21.2) - Chimeric - Azide and BSA Free NBP3-09017-0.2mg from Novus Biologicals, a Bio-Techne Brand

Product Specs
ItemMHC Class I Antibody (R1-21.2) - Chimeric - Azide and BSA Free
CompanyNovus Biologicals, a Bio-Techne Brand
Price $499.00Supplier Page View Company Product Page
Catalog NumberNBP3-09017-0.2mg
Antibody TypePrimary Antibodies
ClonalityMonoclonal
ReactivityMouse
FormatPBS
ApplicationsImmunohistochemistry,Immunoprecipitation,Western Blot,Immunohistochemistry-Paraffin,Radioimmunoassay,Immunocytochemistry/ Immunofluorescence
CloneR1-21.2
Quantity0.2 mg (also 0.025 mg)
ImmunogenThis antibody was raised by immunising Lewis 1F rats with mouse CH-1 lymphoma cells. Spleen cells from immunised rats were fused with X-63Ag8-653 myeloma cells to generate hybridomas
Research AreaAdaptive Immunity, Asthma, Cell Biology, Core ESC Like Genes, Diabetes Research, DNA Repair, DNA replication Transcription Translation and Splicing, Immunology, Stem Cell Markers
TargetMHC Class I
IsotypeIgG Kappa
Conjugate/TagUnconjugated
HostRabbit
PurityProtein A purified
Concentration1 mg/ml
NCBI Full Gene Namemajor histocompatibility complex, class I, E
NCBI Gene AliasesHLA-6.2, QA1
StorageStore at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles

Specifications/Features

Available conjugates: Unconjugated

Questions and Answers

Can this antibody be used for immunohistochemistry in mouse models?

Yes, the MHC Class I Antibody (R1-21.2) has been shown to be effective for immunohistochemical staining in mouse tumor tissues, as demonstrated in studies where it was used to evaluate MHC-I expression in formalin-fixed paraffin-embedded sections ().

How does this antibody perform in the context of immune checkpoint inhibitors?

The antibody has been utilized in research that indicates dietary methionine restriction increases MHC-I expression, which is associated with improved responses to immune checkpoint inhibitors in colorectal cancer models. This suggests that the antibody can be instrumental in studies examining immune responses and therapeutic efficacy ().

Is this antibody suitable for use in flow cytometry applications?

The MHC Class I Antibody (R1-21.2) has not been explicitly mentioned in the context of flow cytometry in the provided literature. Therefore, I do not have information regarding its suitability for flow cytometry applications.

What specific mouse strains or models have been used with this antibody?

The antibody has been referenced in studies involving immunocompetent C57BL/6 mice, particularly in the context of evaluating immune responses in colorectal cancer models and autoimmune conditions like experimental autoimmune encephalomyelitis (, ).

Novus Biologicals, a Bio-Techne Brand

United States: 10771 E Easter Ave

Centennial, Colorado 80112

United States

Phone:
303-730-1950
888-506-6887

Email: novus@novusbio.com

Company Profile

Website: https://www.novusbio.com

(1) Ursolic acid derivative UAOS-Na treats experimental autoimmune encephalomyelitis by immunoregulation and protecting myelinFront NeurolNovember 30, 2023Maolin Wang, Chenming Gu, Yifu Yang, Liang Chen, Kaixian Chen, Jun Du, Huali Wu, Yiming Lialbumin (BSA, #MB4219; Meilunbio) for 1 h. Primary Abs, major histocompatibility complex class I (MHC-I, #NBP3-09017; NOVUS, Beijing, China; 1: 300) and CD8a (#GB114196; Servicebio; 1: 5000), incubations were conducted

(2) Increased Response to Immune Checkpoint Inhibitors with Dietary Methionine Restriction in a Colorectal Cancer ModelCancersSeptember 7, 2023Morehead LC, Garg S, Wallis KF, Simoes CC, Siegel ER, Tackett AJ, Miousse IR.Cell Signaling Technology), rat anti-CD8 (14-0808-80, 1:50, Invitrogen, Waltham, MA, USA), and rabbit anti-MHC-1 (NBP3-09017, 1:50, Novus Biologicals, Centennial, CO, USA) antibodies overnight at 4 °C. This was followed by a 30

(3) Endothelial mechanical stretch regulates the immunological synapse interface of renal endothelial cells in a sex-dependent mannerAmerican journal of physiology. Renal physiologyJuly 1, 2023Colvert CA, Hawkins KP, Semenikhina M, Stefanenko M, Pavlykivska O, Oates JC, DeLeon-Pennell KY, Palygin O, Van Beusecum JP.

Be the First to Write a Review!