Fig 2: a RT-PCR detection showed that the ca group showed high expressions of GRN, PLXNC1 and ORMDL3 compared with the cap group, and the difference between GRN and ORMDL3 was statistically significant (P < 0.05). b WB showed that the expressions of GRN and ORMDL3 were significantly increased in the ca group and the cap group (P < 0.05) and PLXNC1 increased (P > 0.05), but there was no significant difference. c IHC images of human tumor tissue and para-tumor tissue stained with H&E, GRN, PLXNC1 and ORMDL3 antibodies (magnification:200X; scale bar:50 μm). ca: ICC tissues,cap: peritumoral tissues.*P < 0.05, **P < 0.01, ***P < 0.001

Fig 3: The analysis focused on the diagnostic performance and expression levels of the biomarkers. The ROC curves were analyzed for three biomarkers, namely GRN, PLXNC1, and ORMDL3, in GSE107943 (a), TCGA-CHOL (b), and E-MTAB-6389 (c). The box plot compares the expression levels of GRN, PLXNC1, and ORMDL3 in GSE107943 (d), TCGA-CHOL (e), and E-MTAB-6389 (f)

Fig 4: The single gene GSEA on the basis of KEGG gene set of GRN (a), PLXNC1 (b) and ORMDL3 (c)

Fig 5: The process begins with the establishment of the nomogram. a The construction of the nomogram based on GRN, PLXNC1, and ORMDL3. b The calibration curve of nomograms. c The decision curve analysis (DCA) of nomograms