Fig 1: LIGHT promoted BMDM M2 polarization in which PI3Kγ and SGK1 activation are indispensable. a Representative morphological images of BMDMs from the different groups. b Supernatant protein levels of TNF-α, IL-1β, TGF-β1, and IL-10 in different groups. c mRNA levels of IL-10, TGF-β1, ARG1, and CD163 in different groups. d Western blot images of SGK1, AKT, phospho-AKT, PI3Kγ, ARG1, CD163, CD206, and TGF-β1, and e relative expression in the different groups. f Representative fluorescent images of CD163 and the relative fluorescence intensity in different groups. g Representative FCM images and the proportion of CD206-positive macrophages in different groups. *p < 0.05, **p < 0.01. IPI: a PI3Kγ inhibitor, IPI549; GSK: a SGK1 inhibitor, GSK650394
Fig 2: The SGK1 and PI3Kγ pathways were activated in the LIGHT-stimulated heart. a Volcano plot of 661 differentially expressed genes. b GO and KEGG enrichment analyses of 661 differentially expressed genes. c Western blot images of SGK1, AKT, phospho-AKT, and PI3Kα/γ, and d relative expression in the heart samples normalized to the control. *p < 0.05, **p < 0.01. SGK1: serum and glucocorticoid-inducible kinase 1; AKT: protein kinase B; PI3Kα/γ: phosphoinositide-3 kinase α/γ
Supplier Page from ABclonal Technology for SGK1 Rabbit mAb