Fig 1: Characteristics of Hmgn3-OE iTSCs(A) IF of TSC markers (CDX2, EOMES and TFAP2C) in Hmgn3-OE iTSCs and WT TSCs. DNA was stained with Hoechst 33342 (5 µg/mL Hoechst 33342 staining solution (diluted in PBS)). Scale bar, 50 µm.(B) Morphological images of cell cultures in bright field of differentiated cells from Hmgn3-OE iTSCs. The differentiated cells from WT-TSCs are the control. The blue arrow indicates syncytiotrophoblast cells (STCs), and the red arrow indicates trophoblast giant cells (TGCs). Scale bar, 100 µm.(C) IF of STC marker (TPBPA), STB marker (SDC1) and TGC marker (GCM1) in the randomly differentiated derivatives from iTSCs. DNA was stained with Hoechst 33342. Scale bar, 50 µm.(D) DNA content analysis of Hmgn3-OE iTSCs during random differentiation on Days 0, 2, 4 and 6. The diploid (2n), tetraploid (4n), and octaploid (8n) DNA contents are indicated. The differentiation of WT-TSCs was used as a control.(E) IHC analysis of hemorrhagic lesions derived from Hmgn3-OE iTSCs and WT-TSCs in vivo for the endothelial marker CD31 and the trophoblast markers TFAP2C, SDC1 and TPBPA. Scale bar, 20 µm.