Fig 1: Analyses of genome-wide chromatin landscape and enhanced TF-binding events in INN-treated groups during mesoderm differentiation. (A) Correlation of differential peaks in control and INN-treated groups of two cell lines during cardiomyocyte differentiation. (B) Median differential chromatin accessibility at TF motifs independently identified across H1 and C15 cell lines. HNF1B, SOX10, and NFIC show enhanced footprint accessibility in INN-treated cells on day2. (C–E) Left, Plotting of expression values (i.e., fragments per kilobase of transcript per million mapped reads [FPKMs]) of differential genes in close spatial proximity to the genomic binding sites of these TFs (specifically, within a moving window of 1500-bp distance to the transcription start site [TSS]). Middle, ATAC-seq signals (i.e. open chromatin peaks) around the center of TF-binding sites across the whole genome in the treatment group is higher than that in control. Right, Top enriched gene ontology (GO) terms of the overlapping genes that were likely regulated by the TFs in both H1 and C15. The red dotted line denotes statistical significance (FDR <0.05) (F) Protein expression of HNF1B, SOX10, and NFIC in INN-treated and control cells on day2 using Western blot analysis. These TFs showed an up-regulation in INN-treated cells compared to that in control. Full-length blots of these cropped images are presented in the Supplementary Figure 7.