Immunocytochemichal analysis of CLIP2 in A549 cells using a Polyclonal antibody (Product # PA5-113370) at a dilution of 1:166. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L). Cells were also counter-stained with DAPI.

Western Blot analysis of CLIP2 in multiple sample types using a CLIP2 polyclonal antibody (Product # PA5-113370) at a concentration of 3.2 µg/ml. A goat anti rabbit polyclonal secondary antibody was used at a dilution of 1:50,000. Sample types that were tested include: Rat brain tissue, Mouse brain tissue .