Western Blot analysis of RUVBL1 performed on various cell lines using a RUVBL1 monoclonal antibody (Product # MA5-35669) at a dilution of 1:1,000. An HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was applied at a dilution of 1:10,000.

Western blot analysis of RUVBL1 in extracts of various cell lines. Samples were incubated with RUVBL1 Monoclonal antibody (Product # MA5-35669) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3 min.