Western Blot analysis of RBPJ performed on various cell lines using a RBPJ monoclonal antibody (Product # MA5-35438) at a dilution of 1:1,000. An HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was applied at a dilution of 1:10,000.

Western blot analysis of RBPJ in extracts of various cell lines. Samples were incubated with RBPJ Monoclonal antibody (Product # MA5-35438) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.