Flow cytometry analysis of CD3z in 1X10^6 Raji cells (negative control, left)and Jurkat cells (right). Cells were intracellularly-stained with CD3z monoclonal antibody (Product # MA5-35073) (10 μg/mL, orange line) or Rabbit IgG isotype control (10 μg/mL, blue line), followed by FITC conjugated goat anti-rabbit polyclonal antibody (1:200 dilution) staining. Non-fluorescently stained cells were used as blank control (red line).

Immunohistochemical analysis of CD3z performed on paraffin embedded human colon using a CD3z monoclonal antibody (Product # MA5-35073) at a dilution of 1:100. An HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was applied at a dilution of 1:10,000.