Fig 1: Characterisation of ameloblastoma cells in 3D culture. Spheroid formation of (a) AM-1 cells and (b) AM-3 cells in 3D tumouroids at day 7, red = Phalloidin, blue = DAPI, 20 × Magnification Scale Bar = 50 µm. (c) Histology H&E staining of plexiform patient samples, (d) 3D AM-1 tumouroids, e follicular patient samples, and (f) 3D AM-3 tumouroids. Similar anastomosing cords and branches were highlighted (c, d) 10 × Magnification Scale Bar = 100 µm. The formation of the odontogenic islands was highlighted (e,f). (g) CellTiter-Glo 3D Viability-Assay of AM-1, AM-3 and MG-63 cells in 3D tumouroids. Distance of invasion (µm) AM-1, AM-3 and MG-63 cells from the tumour mass to the surrounding stroma within the 3D tumouroids. (h) Human MMP-2 ELISA. Expression of MMP2. (i) Human MMP-9 ELISA. Expression of MMP9. (j) Expression of TNFRSF11A (RANK). Human RANKL ELISA. One-Way ANOVA, Dunnet’s Post Hoc; p-values 0.05 = *, 0.005 = **, 0.0005 = *** and 0.00005 = ****.
Supplier Page from Abcam for Anti-RANK antibody