Fig 1: Immune Infiltration Analysis and Correlation with Immune Checkpoints. (A) Correlation between RFXANK expression and the infiltration levels of 24 immune cell types. (B) Proportional composition of various immune cell types in the low-expression and high-expression groups of RFXANK. (C) RFXANK was significantly associated with TNFRSF4. (D) RFXANK was significantly associated with PDCD1. (E) RFXANK was significantly associated with TNFRSF18. (F) RFXANK was significantly associated with CTLA4. (G) RFXANK was significantly associated with LAG-3. (H) RFXANK was significantly associated with TIGIT. Significance identifier: *, p < 0.05; ***, p < 0.001.
Fig 2: Correlation between RFXANK Expression and Clinicopathological Parameters. (A–J) Expression of RFXANK in different patient groups with different clinicopathological factors. (K) Overall survival curve of RFXANK from TCGA database. (L) The ROC curve of RFXANK. Significance identifier: *, p < 0.05; ***, p < 0.001.
Fig 3: Subgroup Analysis of RFXANK Expression and Survival Prognosis. (A–I) Kaplan–Meier survival curves of RFXANK expression in relation to overall survival (OS) in different patient subgroups with different clinicopathological factors.
Fig 4: Correlation Analysis. (A) Co-expression heatmap of the top 10 genes positively and negatively correlated with RFXANK. (B,C) Correlation network diagrams of RFXANK with its top 10 positively and negatively correlated genes. (D,E) Heatmaps of immune cell infiltration for RFXANK and its top 10 positively and negatively correlated genes. Significance identifier: *, p < 0.05.
Fig 5: Effects of RFXANK Knockdown on Hepatocellular Carcinoma Cells. (A) Relative mRNA expression levels of RFXANK in normal hepatocyte line LO2 and hepatocellular carcinoma cell lines Huh-7 and MHCC-97H. (B) Knockdown efficiency of RFXANK in Huh-7 cells. (C) Knockdown efficiency of RFXANK in MHCC97H cells. (D,E) Proliferation of Huh-7 and MHCC97H cells assessed by CCK-8 assay. (F) RFXANK protein interaction network diagram. (G–I) Western blot analysis of RAF1 and RFXANK protein levels following RFXANK knockdown in Huh-7 and MHCC97H cells. Significance identifier: **, p < 0.01, ***, p < 0.001. All results were from three independent experiments (n = 3).
Supplier Page from Abcam for Anti-RFXANK antibody [OTI3E7]