Fig 1: The role of RBP4 in EECs and ESCs. A Validation of iTRAQ results of RBP4 by using RT-qPCR. Gray lines and blank column representing the values were determined by iTRAQ and RT-qPCR, respectively. The r-value shows Pearson’s correlation between two sets of values determined by above two methods. B The results of Western blot. C Results of a CCK8 assay after treatment with Si-RBP4 during EECs proliferation. D, E The effects of Si-RBP4 on cell cycle and apoptosis. F, G After treatment with Si-RBP4, cell migration was determined using a transwell assay. In H, relative cell number represents the average cell number found. H, I The effect of Si-RBP4 on the mRNA expression levels of genes related to embryo implantation. J, K Comparison of the number of implantation sites between Si-RBP4 (mmu) injected uterine horn (left, L) and NC-injected uterine horn (right, R) (n = 5). L The expression of RBP4 is presented as a value relative to β-actin (n = 3). Results are mean ± s.d. *P < 0.05, **P < 0.01 (Student’s t-test). Scale bar: 200 µm. NC, negative control