Fig 1: The single-cell landscape of an IDH wild-type glioma. (A) Representative images of H&E staining and IHC staining for GFAP and IDH1 proteins in the glioma tissue. Scale bars, 100 μM. (B) The t-SNE plot showing 5,945 single cells colored by cell types. TAMs, tumor associated macrophages. (C) The t-SNE plot showing the GFAP, MOG, PTPRC, CD68, CD3D and MZB1 expression levels in individual cells. (D) The cell number of each cell type in this glioma was displayed. (E) The t-SNE plot showing IDH1, IDH2, EGFR, TP53, CDK4 and PDGFRA expression levels in individual cells.
Fig 2: The expression of ACAA1, IDH1, PEX3 and DBI in human scalp and the results of dual-luciferase reporter assay. (A) Validation of the expression of ACAA1, IDH1, PEX3 and DBI involved in the lipid metabolism in human scalp tissue from AGA and NC. Quantitative analysis reveals a significant upregulation of ACAA1and PEX3 in scalp tissues from AGA patients compared to NC. In contrasts, IDH1 shows downregulation, while DBI exhibits no significant change. (B) Localization in the hair bulb revealed expression of ACAA1, IDH1, and PEX3 in hair follicle cells, including dermal papilla cells and ORSCs. (C) Localization in the hair bulb revealed expression of DBI in dermal papilla cells without expression of DBI in ORSCs. (D) Binding sites and mutation sites of ACAA1 via miR-1343-3p. In the WT group, co-transfection with hsa-miR-1343-3p mimics and the ACAA1 WT plasmid resulted in a significant decrease in luciferase activity compared to the mimics NC group, with no change observed in the MUT group. (E) Binding sites and mutation sites of IDH1 via miR-3609_R-2. In the WT group, co-transfection with hsa-miR-3609_R-2 mimics and the IDH1 WT plasmid resulted in a significant decrease in luciferase activity compared to the mimics NC group, with no change observed in the MUT group. AGA, androgenetic alopecia; NC, normal controls; ORSCs, outer root sheath cells; WT, the wild-type reporter plasmid; MUT, the mutant reporter plasmid; mimics NC, negative control plasmid; miR-3609_R-2 mimics, overexpressed miR-3609_R-2 plasmid; miR-1343-3p mimics, overexpressed miR-1343-3p plasmid. Scale bars, 312.5 μm. * p < 0.05; ** p < 0.01; *** p < 0.001; ns p > 0.05.
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