Fig 1: TMPRSS2 protein expression is upregulated in the lungs of ozone -exposed mice. Western blot representative gel image (A) showing bands for TMPRSS2 protein and alpha-tubulin loading control, and band intensity analyses (B, Bar Graph) on the whole lung homogenate from air- and ozone-exposed mice (n = 6–8). (C) Immunohistochemical staining for TMPRSS2 in macrophages (solid red arrow), alveolar epithelial cells (solid green arrow), and bronchiolar epithelial cells (solid purple arrow). Negatively stained cells are indicated by dotted arrows in lung sections that were incubated with antibody (IgG) control. All images were captured at the same magnification.
Fig 2: Effect of phenolic compounds on ACE2 and TMPRSS2 expression in HepG2 cells and 293T cells. In different cell lines, (A) hispidin, (B) DBA, (C) PAC, (D) PAD and (E) CA were added and cultured for 24 h and analyzed ACE2 and TMPRSS2 expression by Western blot. After densitometric analysis, the results were expressed as a ratio (SS/control). β-actin was chosen as an inner control. (DBA: 3,4- dihydroxybenzalacetone; PAC: protocatechuic acid; PAD: protocatechualdehyde; CA: caffeic acid.).
Fig 3: SC inhibited the ACE2 and TMPRSS2 expression in the liver (A), kidney (B), and lung (C) tissues in mice. Protein levels of ACE2 and TMPRSS2 expression in the liver and kidney tissues were assessed using Western blot. The densitometric analysis of protein bands is presented as a ratio (SC/control). Data are the means ± SD (n = 3). β-actin was used as a positive control.
Fig 4: Adenosine inhibited the levels of ACE2 and TMPRSS2 expression in HepG2 cells and 293T cells. HepG2 (A) and 293T (B) were treated with adenosine (0.5 and 1.0 mM) for 24 h, and ACE2 and TMPRSS2 expression was measured using Western blot. The densitometric analysis of protein bands is presented as a ratio (SC/control). β-actin was used as the positive control.
Fig 5: Effect of SS on mouse model. (A) The mice were measured weight and counted after fed with 100 mg/kg SS by oral gavage. The images captured from IHC stained in (B) liver and (C) kidney tissue. Representative histological sections after IHC staining were zoomed in at 200 x and photographed for documentation. The results were presented as IOD/area (%). The values are reported as the mean ± S.E.M (n = 6). *p < 0.05, **p < 0.01 and ***p < 0.001 contrast with the control group. Arrows show the expression of ACE2 or TMPRSS2; scale bar = 100 µm. Protein levels of ACE2 and TMPRSS2 protein expression in the (D) liver and (E) kidney tissues were analyzed by Western blot after treated with 100 mg/kg SS. After densitometric analysis, the results were expressed as a ratio (SS/control). β-actin was chosen as an inner control.
Supplier Page from Abcam for Anti-TMPRSS2 antibody