Fig 2: RPL35A was up-regulated in GC and RPL35A knockdown cell model was constructed. A The expression levels of RPL35A in GC tumor tissues and para-carcinoma tissues were determined by immunohistochemical staining. B The knockdown efficiencies of shRPL35A-1, shRPL35A-2 and shRPL35A-3 were detected by qRT-PCR. C The fluorescence expression in cells was observed after 72 h-infection. Magnification times: 200 ×. D The RPL35A expression in GC cell lines after infection was analyzed by qRT-PCR. E The expression of RPL35A protein in GC cell lines after infection was detected by western blot. Results were presented as mean ± SD. *P < 0.05, ***P < 0.001

Fig 3: The effects of RPL35A knockdown on cell apoptosis and cell cycle. A The effects of RPL35A knockdown on cell apoptosis were examined by flow cytometry. B The effects of RPL35A knockdown on cell cycle were determined by flow cytometry. Results were presented as mean ± SD. **P < 0.01, ***P < 0.001

Fig 4: RPL35A knockdown suppressed GC growth in vivo. A L and W of tumors were recorded to calculate the volume of tumors from feeding to sacrifice. B The weight of tumors was measured after sacrificing mice. C The photograph of tumors was taken after removing tumors. D The value of Ki-67 was detected by IHC in tumor sections. Results were presented as mean ± SD. **P < 0.01, ***P < 0.001

Fig 5: RPL35A knockdown inhibited cell proliferation and migration. A The cell proliferation rate was evaluated in GC cell lines after infection by the Celigo cell counting assay. B The migration rate of cells was detected in GC cell lines after infection by wound-healing assay. C The migration rate of cells was detected in GC cell lines after infection by transwell assay. Results were presented as mean ± SD. **P < 0.01, ***P < 0.001