Fig 1: Validation of a SS18-SSX2 fusion demonstrated by the ArcherFusion Plex Sarcoma Panel using alternative methods for fusion detection.(a1) H&E morphology of a monophasic synovial sarcoma with a SS18-SSX2 fusion detected by the AFPSP. (a2) IHC demonstrates a strong nuclear staining with a SS18-SSX fusion specific antibody (clone E9X9V; cat# 72364; Cell Signaling Technology, Danvers, MA, USA). (a3) IHC shows strong nuclear staining with an SSX-specific antibody (clone E5A2C; cat# 23855; Cell Signaling Technology). (b) FISH demonstrating a SS18 rearrangement using the Zyto Light SPEC SS18 Dual Color Break Apart Probe (Zytovision, Bremerhaven, Bremen, Germany). (c) Gel electrophoresis image illustrating the rt-PCR results with confirmation of the SYT-SSX2 fusion product.AFPSP, ArcherFusion Plex Sarcoma Panel; FISH, fluorescence in situ hybridisation; H&E, haematoxylin and eosin; IHC, immunohistochemistry; rt-PCR, real-time polymerase chain reaction.