Fig 2: The miRNA–target gene and target gene–TF regulatory network. (A) Network diagram of miRNAs regulating TOX or RasGRP3. Bright red represents TOX or RasGRP3, green represents miRNAs. (B) Network diagram of TFs regulating 23 significant DEGs. Purple circles indicate 23 significant DEGs, green octagons indicate TFs with targets = 10. (C) Network diagram of TFs simultaneously regulating TOX, RasGRP3, and TSPAN13. Purple circles indicate TOX, RasGRP3, or TSPAN13, green octagons indicate TFs.

Fig 3: HE staining and validation of the microarray data by IHC. (A) Representative figures of HE staining in group A without coronary stenosis. (B) Representative figures of HE staining in group B with coronary stenosis less than 25%. (C) Negative control of TOX in group A. (D) Negative control of TOX in group B. (E) Representative IHC figures of TOX in group A. (F) Representative IHC figures of TOX in group B. Compared to group A, the expression of TOX in group B was significantly upregulated (p = 0.031). (G) Negative control of RasGRP3 in group A. (H) Negative control of RasGRP3 in group B. (I) Representative IHC figures of RasGRP3 in group A. (J) Representative IHC figures of RasGRP3 in group B. Compared to group A, the expression of RasGRP3 in group B was significantly downregulated (p = 0.032). (K) Negative control of TSPAN13 in group A. (L) Negative control of TSPAN13 in group B. (M) Representative IHC figures of TSPAN13 in group A. (N) Representative IHC figures of TSPAN13 in group B. Compared to group A, the expression of TSPAN13 in group B was significantly downregulated (p = 0.046). Magnification of HE: 200×. Magnification of IHC: 400×. bar = 50 µm.