Rabbit (DA1E) mAb IgG XP ® Isotype Control (CUT&RUN) 66362S from Cell Signaling Technology

Product Specs
ItemRabbit (DA1E) mAb IgG XP ® Isotype Control (CUT&RUN)
CompanyCell Signaling Technology
Price $170.00 Inquire
Catalog Number66362S
ClonalityMonoclonal
Quantity100 µl
HostRabbit
Research AreaRelated Products
IsotypeIgG
ApplicationsC&R

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Cell Signaling Technology

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Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

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Website: http://www.cellsignal.com/

Website: Technical Support

(1) Inhibiting LSD1 unlocks retinoid AP-1 programming to activate epithelial immunity and skin tumor suppressionJournal of Clinical InvestigationApril 15, 2026Nina Kuprasertkul, Alyssa F. Moore, Carina A. D'souza, Julia Chini, Eun-Kyung Ko, Sijia Huang, Shuo Zhang, Ashley S. Anderson, Shaun Egolf, Laura V. Pinheiro, et al.ab7766, 2 μL), H3K4me3 (Cell Signaling Technology 9751T, 2 μL), and IgG (Cell Signaling Technology 66362S, 5 μL) (Supplemental Table 12). DNA was extracted with spin columns (Cell Signaling Technology 142095S).

(2) Metformin alleviates aging-associated periodontitis via NRF2-mediated restoration of the IRE1α dependent unfolded protein responseBioact MaterApril 9, 2026Yu Sun, Qifeng Chen, Yan Zhao, Yuanyuan Yin, Qiao Chen, Lixin Yang, Zixuan Liu, Rui Chen, Xiaohui Xu, Jinlin Songpermeabilization using 0.01% freshly dissolved digitonin. Primary antibodies against NRF2 (#3724, CST) and IgG (#66362, CST) were added and incubated at 4 °C for 4 h pAG-Mnase was then added and incubated at 4 °C for 90

(3) Friend of GATA2 Variant Ser657Gly Is Associated With Coronary Microvascular DiseaseCirc Genom Precis MedApril 1, 2026Marie A. Guerraty, Shefali S. Verma, Yi-An Ko, Michael A. McQuillan, Donna M. Conlon, John W. Tobias, Michael G. Levin, William Haury, Fangyuan Zheng, Tess Cherlin, et al.HIF1a (hypoxia-inducible factor; CST no. 36169S and Abcam no. 2185) or control IgG antibodies (CST no. 66362). Sequencing was performed on NovaSeq, and data were analyzed using Partek Flow software (version 10.0.23.0414).

(4) MYRF controls mesothelium specification, signaling, and plasticity in lung developmentDevelopmental CellMarch 11, 2026Gidsela Luna, Jamie M. Verheyden, Chunting Tan, Estelle Kim, Ziai Zhu, Michelle Hwa, Jugraj Sahi, Yufeng Shen, Wendy K. Chung, David J. McCulley, et al.anti-H3K27ac, 1:100",Cell Signaling Technology,8173S "rabbit anti-IgG, 1:20",Cell Signaling Technology,66362S "rabbit anti-pro-SFTPC, 1:200",Millipore,AB3786 "rabbit anti-Scgb1a1,1:200",Seven Hills Bioreagents,WRAB-3950 "rabbit

(5) Epigenetic control of microglial mitochondrial immunity by KAT7 drives Alzheimer’s disease pathogenesisbioRxivFebruary 24, 2026Yongqing Liu, Minghua Fan, Yingzhi Ye, Henry Yi Cheng, Shuying Sun, Zhaozhu Qiuagainst H3K14ac (rabbit, Cell Signaling Technology, #7627) and IgG (rabbit, Cell Signaling Technology, #66362) at 4 °C overnight. On the next day, samples were incubated with goat anti-rabbit secondary antibodies

(6) Epigenetic and Transcriptional Programs Define Osteosarcoma Subtypes and Establish Targetable VulnerabilitiesCancer DiscoveryFebruary 6, 2026Eunice López-Fuentes, Andrew S. Clugston, Alex G. Lee, Leanne C. Sayles, Natalie Sorensen, Maria V. Pons Ventura, Stanley G. Leung, Truc Dinh, Marcus R. Breese, E. Alejandro Sweet-Cordero(Cell Signaling Technology, #9751, RRID: AB_2616028), or an IgG antibody control (Cell Signaling Technology, #66362, RRID: AB_2924329) at 4°C. Cells were then washed with digitonin buffer (Cell Signaling Technology,

(7) Clostridioides difficile TcdB induces expression of its receptor (CSPG4) through a noncanonical Hippo signaling mechanismJournal of Biological ChemistryFebruary 1, 2026Jason L. Larabee, Elizabeth J. Donald, Anushka A. Sukhadia, Tyler M. Shadid, Sarah J. Miller, Jimmy D. Ballard(Cell Signaling Technology; product # 9751); and a rabbit monoclonal isotype control (Cell Signaling Technology; product # 66362). DNA was isolated from 2 separate CUT&RUN assays for each condition. Sequencing libraries were constructed

(8) Altered histone modifications in Aedes aegypti midguts following Rift Valley fever virus exposureScientific ReportsJanuary 29, 2026Hunter A. Ogg, Zoey M. Mikol, David C. King, Chad E. Mire, Zeyad Arhouma, Erin Osborne Nishimura, Rebekah C. Kading, Corey L. Campbellreaction anti-H3K9me3 Ab (Abcam, ab8898) and Rabbit (DA1E) mAb IgG XP® Isotype Control (Cell Signaling #66362) as a negative control. Samples were incubated overnight at 4 °C on a rotator. Then the samples were

(9) SARS-CoV-2 Nonstructural Protein 1 Suppresses Host Transcription by Reducing RNA Polymerase II LevelsiScienceDecember 19, 2025Jianfang Li, Kang Wang, Jie Wang, Caizhou Zhong, Shenglan Wang, Hong Peng, Zheng Li(ab284631, Abcam), Lamin A/C (4777, CST), Pol II (05-623, Sigma Aldrich), Flag (14793, CST), and Rabbit IgG (66362, CST). Immunoprecipitation Following transfection, H1299 cells were harvested, washed once with ice-cold

(10) Transcriptional interferences ensure one olfactory receptor per ant neuronNatureDecember 11, 2025Bogdan Sieriebriennikov, Olena Kolumba, Aurore de Beaurepaire, Jennifer Wu, Valentina Fambri, Eva Bardol, Yuwei Zhong, Ildar Gainetdinov, Danny Reinberg, Hua Yan, et al.Grade,abcam,Cat#ab9050 Rabbit (DA1E) mAb IgG XP Isotype Control (CUT&RUN),Cell Signaling Technology,Cat#66362 Critical commercial assays,Critical commercial assays,Critical commercial assays Chromium Next GEM

(11) The formation and propagation of human Robertsonian chromosomesNatureNovember 27, 2025Leonardo Gomes de Lima, Andrea Guarracino, Sergey Koren, Tamara Potapova, Sean McKinney, Arang Rhie, Steven J. Solar, Chris Seidel, Brandon L. Fagen, Brian P. Walenz, et al.1:50 dilution as a positive control, while a rabbit IgG XP isotype control (Cell Signaling Technology, 66362, DA1E monoclonal antibody) was applied at 1:10 dilution as a negative control. Antibody incubation was

(12) Nelfb promotes dermal white adipose tissue formation through RNA Polymerase II mediated adipogenic gene regulationDevelopmentOctober 15, 2025Samiksha Mahapatra, Julian Gomez, Uyanga Batzorig, Ye Liu, Celia Fernández-Méndez, Yifang Chen, George L. Sen#13499S); Nelfe: 5 μl/reaction (Proteintech, #10705-1-AP); IgG: 5 μl/reaction (Cell Signaling Technology, #66362); H3K4me3: 5 μl/reaction (Cell Signaling Technology, #9751S); H3K27me3: 2 μl/reaction (Cell Signaling Technology

(13) SAA1/FPR2 signaling between keratinocytes and neutrophils sustains chronic inflammation in Sweet syndromeJournal of Clinical InvestigationOctober 15, 2025Jianhe Huang, Satish Sati, Olivia Ahart, Emmanuel Rapp-Reyes, Linda Zhou, Robert G. Micheletti, William D. James, Misha Rosenbach, Thomas H. LeungCell Signaling Technology), S100A9 (34425S, Cell Signaling Technology), MHC-II (68258S, Cell Signaling Technology), IgG (66362S, Cell Signaling Technology), NE (AB68672, Abcam), ELANE (LS-B4244, LS-Bio), FPR2 (NLS1878, Novus), SAA1

(14) Coronary artery disease-associated variants regulate vascular smooth muscle cell gene expressionNature Cardiovascular ResearchOctober 7, 2025Nicolas Barbera, Lily Lei, Alexia Wallace, Faruk Erin, R. Noah Perry, Hester M. den Ruijter, Mete CivelekAnti-Acetyl-Histone H3 (Lsy 27) (CST, #8173), 2 uL of Anti-H2BK20ac (#34156), or rabbit IgG (CST, #66362), rotated overnight at 4°C, resuspended in 250 μL of antibody buffer and 7.5 μL of the pAG-MNase enzyme

(15) Gene regulatory mechanisms guiding bifurcation of inhibitory and excitatory neuron lineages in the mouse anterior brainstemeLifeSeptember 12, 2025Sami Kilpinen, Lassi Virtanen, Silvana Bodington Celma, Amos Bonsdorff, Heidi Heliölä, Kaia Achim, Juha PartanenBioSite, ASJ-IO4DE3-50), rabbit anti-Tead2 (Biorbyt, orb382464), rabbit anti-IgG (Cell Signalling, 66362), and rabbit anti-H3K4me3 (Cell Signalling, 9751). The anti-Ebf1, anti-Tal1, anti-IgG, and anti-H3K4me3

(16) Epigenetic dysregulation of energy homeostasis drives aortic valve stenosis that is treatable with metforminJCI InsightSeptember 9, 2025Timothy J. Cashman, Sherin Saheera, Ashley E. Blau, Edith Mensah Otabil, Nouran Y. Nagy, Thomas D. Samenuk, Timothy P. Fitzgibbons, David D. McManus, Chinmay M. Trivediantibodies targeting p-HDAC3, HDAC3, H3K27ac, or IgG control (Cell Signaling Technologies, catalog 66362S). Indexed libraries were prepared and sequenced on an Illumina MiSeq platform with 25 bp paired-end

(17) NF1 Loss Promotes EGFR Activation and Confers Sensitivity to EGFR Inhibition in NF1-Mutant MelanomaCancer ResearchSeptember 2, 2025Milad Ibrahim, Irineu Illa-Bochaca, George Jour, Eleazar Vega-Saenz de Miera, Joseph Fracasso, Kelly Ruggles, Iman Osman, Markus Schober8173 (RRID: AB_10949503)], or Rabbit (DA1E) mAb IgG XP Isotype Control [Cell Signaling Technology, 66362 (RRID: AB_2924329)] antibodies were added at a final 1:50 dilution. Antibodies and permeabilized cells

(18) EBNA leader protein orchestrates chromatin architecture remodeling during Epstein-Barr virus-induced B cell transformationNucleic Acids ResJuly 2, 2025Davide Maestri, Lisa B Caruso, Jana M Cable, Rachel Sklutuis, Sarah Preston-Alp, Robert E White, Micah A Luftig, Italo Temperaantibody (Active Motif 61 779), 1 ug of CTCF antibody (Active Motif 61 311) or 1 ug of IgG antibody (CST 66362S) was added to the reaction and incubated o/n on a nutator at 4°C with cap elevated. Next day beads were

(19) BMAL1 and ARNT enable circadian HIF2α responses in clear cell renal cell carcinomaNature communicationsJuly 1, 2025Mello RM, Gomez Ceballos D, Sandate CR, Wang S, Jouffe C, Agudelo D, Uhlenhaut NH, Thomä NH, Simon MC, Lamia KA.antibodies used for immunoprecipitation were 5 μg of rabbit mAb IgG isotype as a negative control (CST #66362, Lot 3), 2 μg of rabbit mAb tri-methyl-lys-4 (CST # C42D8, Lot 15), 1 μg of HIF-2α rabbit mAb (CST #59973,

(20) JMJD3-mediated senescence is required to overcome stress-induced hematopoietic defectsEMBO ReportsJune 25, 2025Yuichiro Nakata, Takeshi Ueda, Yasuyuki Sera, Miho Koizumi, Katsutoshi Imamura, Akinori Kanai, Ken-ichiro Ikeda, Norimasa Yamasaki, Akiko Nagamachi, Kohei Kobatake, et al.,Cell Signaling,Cat#3457 Rabbit anti-p16INK4a for WB,Cell Signaling,Cat#4824 Rabbit anti-IgG for CUT&RUN,Cell Signaling,Cat#66362 Bacterial and virus strains,Bacterial and virus strains,Bacterial and virus strains Stbl2 Competent

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Rabbit (DA1E) mAb IgG XP ® Isotype Control (CUT&RUN) from Cell Signaling Technology

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Cell Signaling Technology

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Rabbit (DA1E) mAb IgG XP ® Isotype Control (CUT&RUN) from Cell Signaling Technology

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