Fig 1: Absence of GSDMA3 does not affect the expression of pyroptosis associated molecules in B cells(A) Real-time PCR was used to detect the expression of Nlrp3, Caspase 1, Gsdmd, IL1ß, and IL18 in B cells stimulated with LPS (500 ng/mL) for 4 h, followed with Nigericin (10 µM) for 2 h.(B) The expression of NLRP3, GSDMD, CASPASE 1 and IL1ß was detected with Western blot.(C) LDH release was measured in B cells stimulated with LPS and Nigericin. The data were expressed as mean ± SEM (n = 5) from three independent experiments, *p < 0.05, **p < 0.01 (Student’s t test).