Western Blot: B-Raf [p Ser445] Antibody [NBP3-05377] - Western blot analysis of extracts of Raji cells, using B-Raf pAb (NBP3-05377) at 1:2000 dilution or BRAF antibody (A0038).Raji cells were treated by PMA/TPA (200nM) for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 10s.