Western blot analysis of RAB11FIP3 on different lysates with Rabbit anti-RAB11FIP3 antibody (ER1902-80) at 1/5,000 dilution. Lane 1: U-87 MG cell lysate Lane 2: AGS cell lysate Lane 3: Mouse liver tissue lysate Lane 4: Mouse brain tissue lysate Lane 5: Rat liver tissue lysate Lane 6: Rat brain tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 119 kDa Observed band size: 119 kDa Exposure time: 18 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1902-80) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.