M-CSF Biotinylated Antibody BAF416 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemM-CSF Biotinylated Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $559.00Supplier Page View Company Product Page
Catalog NumberBAF416
ApplicationsELISA Development (Detection),Flow Cytometry,Western Blot,ELISA Detection (Matched Antibody Pair)
IsotypeIgG
Conjugate/TagBiotin
TargetM-CSF
ReactivityMouse
HostGoat
FormatLyophilized from a 0.2 µm filtered solution in PBS with BSA as a carrier protein
Quantity50 ug
ClonalityPolyclonal
ImmunogenE. coli-derived recombinant mouse M-CSF (R&D Systems, Catalog # 416-ML), Lys33-Glu262, Accession # Q3U4F9
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution
NCBI Full Gene Namecolony stimulating factor 1
NCBI Gene AliasesCSF-1, MCSF, PG-M-CSF

Specifications/Features

Available conjugates: Available conjugates: Biotin

Specificity: Detects mouse M-CSF in ELISAs and Western blots. In sandwich immunoassays, less than 0.05% cross-reactivity with rhM-CSF is observed.

Sizes Available: 50 ug

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Neutrophil and Macrophage Cell Surface Colony-Stimulating Factor 1 Shed by ADAM17 Drives Mouse Macrophage Proliferation in Acute and Chronic InflammationMolecular and cellular biologySeptember 1, 2018Tang J, Frey JM, Wilson CL, Moncada-Pazos A, Levet C, Freeman M, Rosenfeld ME, Stanley ER, Raines EW, Bornfeldt KE.blocked with 220 ␮l of 1% BSA in PBS at RT. CSF-1 was detected using 0.1 ␮g/ml biotinylated antibody (BAF416; R&D Systems). Following addition of streptavidin-horseradish peroxidase (HRP) and developing reagent (tetramethylbenzidine;

(2) CSF1 receptor targeting in prostate cancer reverses macrophage-mediated resistance to androgen blockade therapyCancer researchMarch 15, 2015Escamilla J, Schokrpur S, Liu C, Priceman SJ, Moughon D, Jiang Z, Pouliot F, Magyar C, Sung JL, Xu J, et al.Duoset Kit (R &D systems) with capture antibody (MAB416, R&D Systems, 2 μg/ml) and detection antibody (BAF416, R&D Systems, 0.2 μg/ml). Tumor models All animal experiments were approved by the Animal Research

(3) Selective deletion of the membrane-bound colony stimulating factor 1 isoform leads to high bone mass but does not protect against estrogen-deficiency bone lossJournal of bone and mineral metabolismJuly 1, 2012Yao GQ, Wu JJ, Troiano N, Zhu ML, Xiao XY, Insogna K.were re-suspended in PBS buffer containing 5% FBS and incubated with biotinylated mouse CSF1 antibody (BAF416, R&D Systems) for 45 min at 4°C. Cells were washed with PBS buffer containing 5% FBS, collected and

(4) Both cell-surface and secreted CSF-1 expressed by tumor cells metastatic to bone can contribute to osteoclast activationExperimental Cell ResearchAugust 15, 2009Kader Yagiz, Susan R Rittlingto the manufacturer’s instructions, the washed plate was then incubated with detection antibody (# BAF416, R&D Systems, 0.2 μg/ml) for 1 h and then with Streptavidin-HRP for 20 min. Optical density of each

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M-CSF Biotinylated Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

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M-CSF Biotinylated Antibody from R&D Systems, a Bio-Techne Brand

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