Human ST2/IL-33R Antibody AF523 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemHuman ST2/IL-33R Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $499.00Supplier Page View Company Product Page
Catalog NumberAF523
ApplicationsFlow Cytometry,Immunohistochemistry,Immunoprecipitation,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Simple Western,Neutralization,Immunocytochemistry,Cell Culture
IsotypeIgG
TargetST2/IL-33R
HostGoat
Conjugate/TagUnconjugated
ReactivityHuman,Mouse
Quantity100 ug (also 25 ug)
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. See Certificate of Analysis for details. *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
ClonalityPolyclonal
ImmunogenS. frugiperda insect ovarian cell line Sf 21-derived recombinant human ST2/IL-33 R, Lys19-Phe328, Accession # BAA02233
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution
NCBI Full Gene Nameinterleukin 1 receptor like 1
NCBI Gene AliasesDER4, FIT-1, IL33R, ST2, ST2L, ST2V, T1

Specifications/Features

Available conjugates: Unconjugated

Questions and Answers

Can this antibody be used to study the role of IL-33 in pulmonary hypertension models?

Yes, the ST2/IL-33R antibody has been utilized in studies investigating IL-33 signaling in pulmonary hypertension. For instance, in a study involving Sugen/hypoxia mice, the antibody was employed to evaluate the role of IL-33/ST2 signaling in endothelial cell proliferation and pulmonary artery remodeling, highlighting its relevance in this context ().

Is this antibody effective for immunohistochemistry in cancer research?

The ST2/IL-33R antibody has been shown to work effectively in immunohistochemistry applications, particularly in studies related to colorectal cancer. It was used to analyze the expression of IL-33 and ST2 in tumor and healthy tissues, providing insights into their roles in tumor microenvironments and metastasis ().

What species can this antibody detect, and how has it been validated in research?

This antibody is reactive with both human and mouse proteins. It has been validated in various applications, including Western blotting and flow cytometry, as demonstrated in multiple studies that utilized the antibody to assess IL-33 and ST2 levels in different experimental setups (, ).

Are there any specific applications where this antibody has been cited in the literature?

Yes, the ST2/IL-33R antibody has been cited in several applications, including Western blotting and immunofluorescence. It has been used to investigate the role of IL-33 in various pathological conditions, such as preeclampsia and gastric cancer metastasis, where it contributed to understanding the signaling pathways involved in these diseases (, ).

R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Lymph node macrophages drive immune tolerance and resistance to cancer therapy by induction of the immune-regulatory cytokine IL-33Cancer CellMay 12, 2025Sara Lamorte, Rene Quevedo, Robbie Jin, Luke Neufeld, Zhe Qi Liu, M. Teresa Ciudad, Sabelo Lukhele, Jessica Bruce, Shreya Mishra, Xin Zhang, et al.gp100 (clone HMB45),Abcam,Cat#ab787;RRID:AB_306146 anti-human ST2 (polyclonal goat),R&D Systems,Cat#AF523;RRID:AB_2125427 anti-mouse Marco (clone ED31),Dr. Mikael Karlsson (Karolinska Institute),10.1016/j.celrep.2016.04.084 goat

(2) A neutrophil elastase-generated mature form of IL-33 is a potent regulator of endothelial cell activation and proliferative retinopathyExperimental & molecular medicineAugust 1, 2024Bisen S, Verma SK, Mukhopadhyay CS, Singh NK.

(3) IL-33/ST2 axis of human amnion fibroblasts participates in inflammatory reactions at parturitionMolecular medicine (Cambridge, Mass.)July 4, 2023Lei WJ, Zhang F, Lin YK, Li MD, Pan F, Sun K, Wang WS.

(4) The IL-33:ST2 axis is unlikely to play a central fibrogenic role in idiopathic pulmonary fibrosisRespiratory researchMarch 23, 2023Stephenson KE, Porte J, Kelly A, Wallace WA, Huntington CE, Overed-Sayer CL, Cohen ES, Jenkins RG, John AE.antibodies used for western blotting included goat anti-IL-33 (AF3625; R&D systems), goat anti-ST2 (AF523; R&D systems), mouse anti-α-Tubulin (TU-02; Santa Cruz Biotechnology, Santa Cruz, CA, USA), rabbit anti-Fibronectin

(5) IL-33/ST2 receptor-dependent signaling in the development of pulmonary hypertension in Sugen/hypoxia micePhysiological reportsFebruary 1, 2022Indralingam CS, Gutierrez-Gonzalez AK, Johns SC, Tsui T, Cannon DT, Fuster MM, Bigby TD, Jennings PA, Breen EC.dilution, Nessy‐1 ALX‐804‐840/1; Enzo Life Sciences, Inc.), ST2 (goat pAb, 1:1000, Human ST2/IL‐33R AF523; R&D Systems), MYD88 (goat pAb, 1:1000, Mouse/Rat MyD88 AF3109; R&D Systems) and α‐tubulin (rabbit Ab,

(6) The reciprocal interaction between tumor cells and activated fibroblasts mediated by TNF-α/IL-33/ST2L signaling promotes gastric cancer metastasisOncogeneFebruary 1, 2020Zhou Q, Wu X, Wang X, Yu Z, Pan T, Li Z, Chang X, Jin Z, Li J, Zhu Z, et al.and TNF-α (no. AF-300-01A) were purchased from PeproTech. Anti-human IL-33 (no. AF3625), ST2L (no. AF523), TNF-α (no. AF-410-NA), TNFR1 (no. MAB225-100) and TNFR2 (no. MAB726-100) neutralizing antibodies were

(7) High level of interleukin-33 in cancer cells and cancer-associated fibroblasts correlates with good prognosis and suppressed migration in cholangiocarcinomaJournal of CancerJanuary 1, 2020Yangngam S, Thongchot S, Pongpaibul A, Vaeteewoottacharn K, Pinlaor S, Thuwajit P, Okada S, Hermoso MA, Thuwajit C.with 1: 500 goat anti-human IL-33 antibody (AF3625, R&D System) or 1:250 goat anti-human ST2 antibody (AF523, R&D System) at 4oC overnight or RT for 2 h. After washing, membrane was incubated with 1:1,000 rabbit

(8) Combinatorial IL-17RB, ST2, and TSLPR Signaling in Dendritic Cells of Patients With Allergic RhinitisFrontiers in cell and developmental biologyJanuary 1, 2020Zheng R, Chen Y, Shi J, Wang K, Huang X, Sun Y, Yang Q.

(9) IL-33 Alarmin and Its Active Proinflammatory Fragments Are Released in Small Intestine in Celiac DiseaseFrontiers in immunologyJanuary 1, 2020Perez F, Ruera CN, Miculan E, Carasi P, Dubois-Camacho K, Garbi L, Guzman L, Hermoso MA, Chirdo FG.USA); for human IL-33 (R&D Systems, Cat# AF3625, RRID: AB_1151900, USA) and ST2 (R&D Systems, Cat# AF523, RRID: AB_2125427, USA); mouse IL-33 (R&D Systems, Cat# AF3626, RRID: AB_884269, USA) and suitable isotypes controls

(10) Interleukin 1 Receptor-Like 1 (IL1RL1) Promotes Airway Bacterial and Viral Infection and InflammationInfection and immunityJuly 1, 2019Schaunaman N, Sanchez A, Dimasuay KG, Pavelka N, Numata M, Alam R, Martin RJ, Chu HW.

(11) Interleukin 33/ST2 Axis Components Are Associated to Desmoplasia, a Metastasis-Related Factor in Colorectal CancerFrontiers in immunologyJanuary 1, 2019Landskron G, De la Fuente López M, Dubois-Camacho K, Díaz-Jiménez D, Orellana-Serradell O, Romero D, Sepúlveda SA, Salazar C, Parada-Venegas D, Quera R, et al.antibody, A2547, Sigma–Aldrich, St. Louis, MO, USA) and anti-ST2 (1/100) (Polyclonal Goat anti human, AF523, R&D Systems, Minneapolis, MN, USA) in conjunction with anti-E-cadherin (1/500) (monoclonal mouse antibody,

(12) IL-33 down-regulates CLDN1 expression through the ERK/STAT3 pathway in keratinocytesJournal of dermatological scienceJune 1, 2018Ryu WI, Lee H, Bae HC, Jeon J, Ryu HJ, Kim J, Kim JH, Son JW, Kim J, Imai Y, et al.

(13) Decreased IL-33 Production Contributes to Trophoblast Cell Dysfunction in Pregnancies with PreeclampsiaMediators of inflammationJanuary 1, 2018Chen H, Zhou X, Han TL, Baker PN, Qi H, Zhang H.IL-33 (12372-1-AP, Proteintech Group Inc., Chicago, USA), goat polyclonal antibody for ST2/IL-1 R4 (AF523, R&D Systems, Minneapolis, USA), rabbit polyclonal antibody for ST2/IL-1 R4 (11920-1-AP, Proteintech Group

(14) IL-33 stimulates CXCL8/IL-8 secretion in goblet cells but not normally differentiated airway cellsClinical and experimental allergy : journal of the British Society for Allergy and Clinical ImmunologyApril 1, 2014Tanabe T, Shimokawaji T, Kanoh S, Rubin BK.

(15) Interleukin-33 drives a proinflammatory endothelial activation that selectively targets nonquiescent cellsArteriosclerosis, thrombosis, and vascular biologyFebruary 1, 2013Pollheimer J, Bodin J, Sundnes O, Edelmann RJ, Skånland SS, Sponheim J, Brox MJ, Sundlisaeter E, Loos T, Vatn M, et al.antibodies against IL-1R1 (IL-1R–specific goat IgG, AF269, R&D systems) or IL-33R (IL-33R–specific goat IgG, AF523, R&D systems) or kinase inhibitors as specified were added 1 hour before cytokine stimulation. Western Blot

(16) Detection of soluble ST2 in human follicular fluid and luteinized granulosa cellsPloS oneJanuary 1, 2013Southcombe JH, Lédée N, Perrier d'Hauterive S, Turner K, Child T, Snider JV, Redman CW, Sargent IL, Granne I.

(17) Measurement of interleukin-33 (IL-33) and IL-33 receptors (sST2 and ST2L) in patients with rheumatoid arthritisJournal of Korean medical scienceSeptember 1, 2011Hong YS, Moon SJ, Joo YB, Jeon CH, Cho ML, Ju JH, Oh HJ, Heo YJ, Park SH, Kim HY, et al.

(18) ST2 and IL-33 in pregnancy and pre-eclampsiaPloS oneJanuary 1, 2011Granne I, Southcombe JH, Snider JV, Tannetta DS, Child T, Redman CW, Sargent IL.

(19) Expression of interleukin 1-like cytokine interleukin 33 and its receptor complex (ST2L and IL1RAcP) in human pancreatic myofibroblastsGutApril 1, 2010Nishida A, Andoh A, Imaeda H, Inatomi O, Shiomi H, Fujiyama Y.

(20) A novel IL-1 family cytokine, IL-33, potently activates human eosinophilsThe Journal of allergy and clinical immunologyJune 1, 2008Cherry WB, Yoon J, Bartemes KR, Iijima K, Kita H.

Be the First to Write a Review!

ST2/IL-33R Antibody

ST2/IL-33R Antibody

ST2/IL-33R Antibody

ST2/IL-33R Antibody

ST2/IL-33R Antibody

Human ST2/IL-33R Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

Questions and Answers

R&D Systems, a Bio-Techne Brand

Citations (22)

Reviews

Human ST2/IL-33R Antibody from R&D Systems, a Bio-Techne Brand

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map