Human EGFR Antibody AF231 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemHuman EGFR Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $519.00Supplier Page View Company Product Page
Catalog NumberAF231
Quantity100 ug (also 25 ug)
ApplicationsCo-Immunoprecipitation,ELISA Development,ELISA Microarray Development,Flow Cytometry,Immunohistochemistry,Immunoprecipitation,Western Blot,Immunohistochemistry-Paraffin,Proximity Ligation Assay,Simple Western,ELISA Capture (Matched Antibody Pair),Immunofluorescence,Immunocytochemistry,CyTOF-ready,Dual RNAscope ISH-IHC Compatible,ELISA Detection
Conjugate/TagUnconjugated
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. See Certificate of Analysis for details. *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
ConcentrationLYOPH
PurityAntigen Affinity-purified
HostGoat
ReactivityHuman,Mouse
TargetEGFR
IsotypeIgG
Antibody TypePrimary Antibodies
ClonalityPolyclonal
ImmunogenMouse myeloma cell line NS0-derived recombinant human EGFR, Leu25-Ser645, Accession # CAA25240
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution
NCBI Full Gene Nameepidermal growth factor receptor
NCBI Gene AliasesERBB, ERBB1, ERRP, HER1, NISBD2, PIG61, mENA

Specifications/Features

Available conjugates: Unconjugated

Questions and Answers

Can this antibody be used for detecting EGFR in human cancer studies?

Yes, the EGFR antibody has been shown to effectively detect human epidermal growth factor receptor in various applications, including Western blotting and immunohistochemistry, which are commonly used in cancer research.

What evidence is there for the specificity of this antibody in different assays?

The antibody has demonstrated specificity for human EGFR with approximately 3% cross-reactivity with recombinant mouse EGFR and less than 0.1% cross-reactivity with recombinant human ErbB2 and ErbB3, making it suitable for studies focused on human EGFR.

How has this antibody been utilized in recent research related to cancer?

In a study by Beauvais et al. (2022), the antibody was used to investigate the interaction of EGFR with syndecan-4 and syndecan-2 in carcinoma cells, revealing insights into mechanisms of cell cycle progression and potential resistance to EGFR inhibitors.

Is there any information on the performance of this antibody in immunofluorescence applications?

Yes, the antibody has been utilized in immunofluorescence assays, as demonstrated in research by Liu et al. (2021), where it was employed to analyze EGFR interactions in triple-negative breast cancer, highlighting its effectiveness in visualizing receptor dynamics in cellular contexts.

R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Intracellular enzymatic reducing systems control receptor tyrosine kinase signaling via PTP1BSci AdvApril 10, 2026Lucia Coppo, Wenchao Zhao, Qing Cheng, Axel Tobias Scholz, Elias S. J. Arnér, Markus Dagnellquantified and compared to total amount of loading as determined from EGFR intensities (R&D Systems, AF231). All the presented quantifications are derived from three independent biological replicates. To avoid

(2) Spatial protein profiling reveals active roles for astrocytes in the chronic active lesion core during multiple sclerosisActa NeuropathologicaNovember 1, 2025Brandon C. Smith, Anthony Chomyk, Maria L. Habean, Benjamin C. Shaw, Rachel A. Tinkey, Bruce D. Trapp, Jessica L. Williams019-19741), SMI31/32 (BioLegend, 801601/801701), ALDH1L1 (Cell Signaling, 85828S), EGFR (R&D Systems, AF231), p44/42 ERK1/2, (Cell Signaling, 4696T), phospho-p90RSK (Cell Signaling, 8753T), 4-1BB (ThermoFisher,

(3) HER1 (EGFR) and/or HER2 Inclusion Potentiates the Antitumor Effect Elicited by a HER3-Specific Monovalent VaccineMolecular cancer therapeuticsAugust 1, 2025Bermudez-Abreut E, Bergado-Báez G, Fundora-Barrios T, Arencibia-Perezleo J, Lopez Medinilla A, Chao L, Sanchez Ramirez B.

(4) Lateral Ventricular Neural Stem Cells Provide Negative Feedback to Circuit Activation Through GABAergic SignalingCellsMarch 13, 2025Naffaa MM, Yin HH.from ThermoFisher Scientific (Catalog #14-5794-82) and Abcam (Catalog #ab211327), and EGRF (Catalog #AF231, R&D Systems, Minneapolis, MN, USA). Additional primary antibodies used were GFAP Chicken Antibody (Catalog

(5) Antitumor activity of PAbs generated by immunization with a novel HER3-targeting protein-based vaccine candidate in preclinical modelsFrontiers in OncologyOctober 16, 2024Ernesto Bermúdez-Abreut, Gretchen Bergado Báez, Melissa Martínez Pestano, Giuseppe Attanasio, Carlos Yordan Gonzales Castillo, Diana Rosa Hernández Fernández, Rydell Alvarez-Arzola, Andrea Alimonti, Belinda Sánchez-Ramírezprimary antibodies: anti-HER3 (AF234, R&D Systems, USA), anti-HER2 (AF1129, R&D Systems), anti-HER1 (AF231, R&D Systems), or ß-actin (MAB8929, R&D Systems). This was followed by incubation with horseradish peroxidase-conjugated

(6) Drug-resistant EGFR mutations promote lung cancer by stabilizing interfaces in ligand-free kinase-active EGFR oligomersNat CommunMarch 19, 2024R. Sumanth Iyer, Sarah R. Needham, Ioannis Galdadas, Benjamin M. Davis, Selene K. Roberts, Rico C. H. Man, Laura C. Zanetti-Domingues, David T. Clarke, Gilbert O. Fruhwirth, Peter J. Parker, et al.AB_1903890), Anti-Phospho-EGF Receptor (Tyr1068, D7A5 CST 3777; RRID: AB_2096270), Anti-EGFR (R & D Systems, AF231; RRID: AB_355220), Anti-EGFR (phospho Y992, EM-12, Abcam ab81440; RRID: AB_1658463), Anti-mouse IgG-HRP

(7) Distinct interactions stabilize EGFR dimers and higher-order oligomers in cell membranesCell ReportsJanuary 1, 2024Krishna C. Mudumbi, Eric A. Burns, David J. Schodt, Zaritza O. Petrova, Anatoly Kiyatkin, Lucy W. Kim, Emma M. Mangiacapre, Irais Ortiz-Caraveo, Hector Rivera Rivera Ortiz, Chun Hu, et al.4407; RRID:AB_331795 "Goat Anti-Human EGFR Polyclonal antibody, Unconjugated",R & D Systems,Cat#: AF231; RRID:AB_355220 Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (E10) Mouse mAb antibody,Cell Signaling Technology

(8) The EGFR phosphatase RPTPγ is a redox-regulated suppressor of promigratory signalingThe EMBO journalMay 15, 2023Joshi MS, Stanoev A, Huebinger J, Soetje B, Zorina V, Roßmannek L, Michel K, Müller SA, Bastiaens PI.(2236, Cell Signaling Technology) rabbit anti‐EGFR (4267, Cell Signaling Technology), goat anti‐EGFR (AF231, R&D Systems), rabbit anti‐phospho‐ERK‐1/2 Thr/Tyr 202/204 (9101, Cell Signaling Technology), mouse

(9) Nanotechnology and machine learning enable circulating tumor cells as a reliable biomarker for radiotherapy responses of gastrointestinal cancer patientsBiosensors and BioelectronicsApril 15, 2023Michael J. Poellmann, Jiyoon Bu, Stanley Liu, Andrew Z. Wang, Steven N. Seyedin, Chandrikha Chandrasekharan, Heejoo Hong, YoungSoo Kim, Joseph M. Caster, Seungpyo Hongfrom MilliporeSigma (St. Louis, MO). The capture antibodies aEPCAM (AF960), aHER2 (AF1129), aEGFR (AF231), along with recombinant human E-Selectin/CD62E chimera protein, CF were obtained from R&D Systems (Minneapolis,

(10) An atlas of late prenatal human neurodevelopment resolved by single-nucleus transcriptomicsNature communicationsDecember 12, 2022Ramos SI, Mussa ZM, Falk EN, Pai B, Giotti B, Allette K, Cai P, Dekio F, Sebra R, Beaumont KG, et al.for 1 h at room temperature and incubated with primary antibodies (1:50 goat anti-EGFR, R&D Systems AF231; 1:50 mouse anti-EGFR, Abcam ab218383; 1:200 rabbit anti-SOX9, Abcam ab185966; 1:50 rabbit anti-OLIG2,

(11) Integrated Analytical System for Clinical Single-Cell AnalysisAdvanced science (Weinheim, Baden-Wurttemberg, Germany)July 1, 2022Peterson HM, Chin LK, Iwamoto Y, Oh J, Carlson JCT, Lee H, Im H, Weissleder R.line 1,EpCAM,Tumor cells,R&D Systems,AF960,Polyclonal,Goat,AF488,A431 2,EGFR,Tumor cells,R&D Systems,AF231,Polyclonal,Goat,AF488,A431 3,HER2,Tumor cells,CST,45332BC,29D8,Rabbit,AF488,BT474 4,ER (ESR1),Tumor

(12) Plasma membrane proteoglycans syndecan-2 and syndecan-4 engage with EGFR and RON kinase to sustain carcinoma cell cycle progressionThe Journal of biological chemistryJune 1, 2022Beauvais DM, Nelson SE, Adams KM, Stueven NA, Jung O, Rapraeger AC.(University of Leuven) and integrin α6β4 (3E1) from Memorial Sloan-Kettering. Anti-Sdc4 (AF2918), EGFR (AF231), RON (AF691 and FAB6647F), pY1238/1239 RON (AF1947), ITGB4 (mAb 422325) Sdc2 (305515) were from R&D Systems

(13) Glioblastoma mutations alter EGFR dimer structure to prevent ligand biasNatureFebruary 1, 2022Hu C, Leche CA 2nd, Kiyatkin A, Yu Z, Stayrook SE, Ferguson KM, Lemmon MA.

(14) Developmental Origins of Human Cortical Oligodendrocytes and AstrocytesNeuroscience bulletinJanuary 1, 2022Yang L, Li Z, Liu G, Li X, Yang Z.anti-EGFR (1:500, R&D System, BAF1280, for mouse brain sections), goat anti-EGFR (1:1,000, R&D System, AF231, for human brain sections), sheep anti EOMES (1:1,000, R&D System, AF6166); rabbit anti EOMES (1:1,000,

(15) EGFR transactivates RON to drive oncogenic crosstalkeLifeNovember 25, 2021Franco Nitta C, Green EW, Jhamba ED, Keth JM, Ortiz-Caraveo I, Grattan RM, Schodt DJ, Gibson AC, Rajput A, Lidke KA, et al.4267 RRID:AB_2246311,Clone D38B1 WB (1:2000) antibody,Anti-EGFR (Goat polyclonal),R&D Systems,Cat# AF231 RRID:AB_355220,WB (1:1000) Used when blotting for EGFR-Δ998 antibody,Anti-EGFR (Rabbit monoclonal),Cell Signaling Technology

(16) Evaluation of the Targeting and Therapeutic Efficiency of Anti-EGFR Functionalised Nanoparticles in Head and Neck Cancer Cells for Use in NIR-II Optical WindowPharmaceuticsOctober 9, 2021Egnuni T, Ingram N, Mirza I, Coletta PL, McLaughlan JR.for 1 h. Cells were then incubated with 1 µg/mL of primary anti-human EGFR goat polyclonal antibody (#AF231, R&D Systems) or a biotinylated anti-human IgG1 rabbit monoclonal antibody (#31-1019-02, 2BScientific)

(17) Bacterial Antigens Reduced the Inhibition Effect of Capsaicin on Cal 27 Oral Cancer Cell ProliferationInternational journal of molecular sciencesAugust 12, 2021Chakraborty R, Vickery K, Darido C, Ranganathan S, Hu H.

(18) Involvement of cancer-derived EMT cells in the accumulation of ¹⁸F-fluorodeoxyglucose in the hypoxic cancer microenvironmentScientific reportsMay 17, 2021Sugita S, Yamato M, Hatabu T, Kataoka Y.Santa Cruz Biotechnology; cat. no. sc-101454), human EGFR (A431 derived tumor cell marker, 1:200; R&D systems; cat. no. AF231), human S100A4 (1:50; Acris Antibodies; cat. no. AP16925PU-N), and mouse S100A4 (1:50; R&D systems;

(19) Combined Bacterial Antigen Lipopolysaccharide and Lipoteichoic Acid Increase Cal 27 Oral Cancer Cell ProliferationDental Oral Biology and Craniofacial ResearchFebruary 8, 2021Shoba Ranganathan, Rajdeep Chakraborty, Honghua Hu, Abubakar Siddiq Mangani, Karen Vickery, Shoba Ranganathan#MAB2686); 0.1 µg/ml STAT3 Mouse anti-human, mouse, rat (Cat #MAB1799); 1 µg/ml human EGFR antibody (Cat #AF231), 1:1000 human GAPDH (Cat #2275-PC-020)]. Blots were probed with the respective secondary antibodies

(20) EGFR inhibition blocks cancer stem cell clustering and lung metastasis of triple negative breast cancerTheranosticsJanuary 1, 2021Liu X, Adorno-Cruz V, Chang YF, Jia Y, Kawaguchi M, Dashzeveg NK, Taftaf R, Ramos EK, Schuster EJ, El-Shennawy L, et al.primary antibodies, anti-Flag (1:1000, Sigma-Aldrich, F1804-200UG) or anti-EGFR (1:1000, R&D Systems, AF231), for 1 h at room temperature or 4 °C overnight. After two washes with TBS/0.1% Tween 20, the membrane

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