Anti-Ribonuclease Inhibitor/RNH1 Antibody Picoband® (monoclonal, 4F3) M04147 from BosterBio

Supplier Images

Anti-Ribonuclease Inhibitor/RNH1 Antibody Picoband™ (monoclonal, 4F3)

Western blot analysis of Ribonuclease Inhibitor/RNH1 using anti-Ribonuclease Inhibitor/RNH1 antibody (M04147).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human 293T whole cell lysates,
Lane 4: human placenta tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Ribonuclease Inhibitor/RNH1 antigen affinity purified monoclonal antibody (Catalog # M04147) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Ribonuclease Inhibitor/RNH1 at approximately 45 kDa. The expected band size for Ribonuclease Inhibitor/RNH1 is at 50 kDa.
Anti-Ribonuclease Inhibitor/RNH1 Antibody Picoband (monoclonal, 4F3)

Anti-Ribonuclease Inhibitor/RNH1 Antibody Picoband (monoclonal, 4F3)
Anti-Ribonuclease Inhibitor/RNH1 Antibody Picoband™ (monoclonal, 4F3)

IHC analysis of Ribonuclease Inhibitor/RNH1 using anti-Ribonuclease Inhibitor/RNH1 antibody (M04147).
Ribonuclease Inhibitor/RNH1 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-Ribonuclease Inhibitor/RNH1 Antibody (M04147) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
Anti-Ribonuclease Inhibitor/RNH1 Antibody Picoband™ (monoclonal, 4F3)

IHC analysis of Ribonuclease Inhibitor/RNH1 using anti-Ribonuclease Inhibitor/RNH1 antibody (M04147).
Ribonuclease Inhibitor/RNH1 was detected in paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-Ribonuclease Inhibitor/RNH1 Antibody (M04147) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.

Supplier Page from
BosterBio for
Anti-Ribonuclease Inhibitor/RNH1 Antibody Picoband® (monoclonal, 4F3)

BosterBio's Anti-Ribonuclease Inhibitor/RNH1 Antibody Picoband® (monoclonal, 4F3) is a Mouse monoclonal antibody. The Anti-Ribonuclease Inhibitor/RNH1 Antibody Picoband® (monoclonal, 4F3) was generated using RNH1 as the antigen. It reacts with Homo Sapiens, and Human. This antibody has been shown to work in applications such as: Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, and Western Blot.

Description

Boster Bio Anti-Ribonuclease Inhibitor/RNH1 Antibody Picoband® (monoclonal, 4F3) catalog # M04147. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance

Anti-Ribonuclease Inhibitor/RNH1 Antibody Picoband™ (monoclonal, 4F3)

Anti-Ribonuclease Inhibitor/RNH1 Antibody Picoband (monoclonal, 4F3)

Anti-Ribonuclease Inhibitor/RNH1 Antibody Picoband™ (monoclonal, 4F3)

Anti-Ribonuclease Inhibitor/RNH1 Antibody Picoband™ (monoclonal, 4F3)

Anti-Ribonuclease Inhibitor/RNH1 Antibody Picoband® (monoclonal, 4F3) from BosterBio

Description

BosterBio

Reviews

Anti-Ribonuclease Inhibitor/RNH1 Antibody Picoband® (monoclonal, 4F3) from BosterBio

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map