Fas/TNFRSF6/CD95 Antibody (R-125224) [PerCP] NBP2-81112PCP from Novus Biologicals, a Bio-Techne Brand

Product Specs
ItemFas/TNFRSF6/CD95 Antibody (R-125224) [PerCP]
CompanyNovus Biologicals, a Bio-Techne Brand
Price $549.00Supplier Page View Company Product Page
Catalog NumberNBP2-81112PCP
Quantity0.1 ml
ApplicationsFlow Cytometry,ELISA,Functional
Conjugate/TagPerCP
FormatPBS
PurityProtein A purified
ReactivityHuman
TargetFas/TNFRSF6/CD95
IsotypeIgG1 Kappa
CloneR-125224
HostHuman
ImmunogenR-125224 is generated by the humanization of the murine HFE7A anti-Fas/TNFRSF6/CD95 antibody by grafting the CDR regions to the framework regions of the human 8E10 antibody and substituting key framework residues from the murine antibody into the 8E10 sequence. The original HFE7A was derived from a hybridoma cell line generated by the fusion of NS1 myeloma cells with splenocytes from Fas-deficient mice which had been immunized with partially purified recombinant human Fas-AIC2A chimera protein consisting of the extracellular region of human Fas/TNFRSF6/CD95 antigen (aa -16 to 150) and the extracellular region of the murine IL-3 receptor AIC2 (aa 3-423). The HFE7A hybridoma was selected after screening by flow cytometry for the production of antibodies with the ability to bind to the WR19L12a transformed murine T cell lymphoma cell line expressing human Fas/TNFRSF6/CD95 or the L5178YA1 cell line expressing murine Fas/TNFRSF6/CD95, but not to the parental WR19L or L5178Y cells
ClonalityMonoclonal
Antibody TypePrimary Antibodies
Research AreaApoptosis, Cancer, Cell Biology, Death Receptor Signaling Pathway, Diabetes Research, Hematopoietic Stem Cell Markers, Immunology, Inhibitors Activators and Regulators, Innate Immunity, Signal Transduction, Stem Cells
NCBI Full Gene NameFas cell surface death receptor
NCBI Gene AliasesALPS1A, APO-1, APT1, CD95, FAS1, FASTM, TNFRSF6
StorageStore at 4C in the dark

Specifications/Features

Available conjugates: Available conjugates: PerCP

Specificity: R-125224 binds to the extracellular portion of human Fas/TNFRSF6/CD95 at an eptiope consisting of the sequence RTQNTKCRCK (aa 105-114) (pmid: 11754745). Fas is a type I membrane protein which belongs to the tumor necrosis factor (TNF) receptor/nerve growth factor (NGF) receptor superfamily. It is able to transduce apoptotic signals into the cell when bound by its ligand FasL (Fas ligand), which is primarily expressed in activated T lymphoid-myeloid lineage cells, in the eye, in reproductive organs and in some tumors. The Fas-FasL system is known to play an important role in maintaining the immune system as mice with Fas-defective lymphoproliferation (lpr) and FasL-defective generalized lymphoproliferative disease (gld) mutations develop massive lymphadenopathy and autoimmune diseases.

Sizes Available: 0.1 ml

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