Western blot analysis of PCDH17 in extracts of 293T cells. Samples were incubated with PCDH17 Polyclonal antibody (Product # PA5-110077) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.