Western blot analysis of GSDMB in extracts of HT-29 cells. Samples were incubated with GSDMB Polyclonal antibody (Product # PA5-109791) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.