Immunocytochemistry analysis of ELOA in MCF7. Samples were incubated in polyclonal ELOA antibody (Product # PA5-110466) using a dilution of 1:50.

Western blot analysis of ELOA in extracts of various cell lines. Samples were incubated with ELOA Polyclonal antibody (Product # PA5-110466) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.