Western blot analysis of Nectin 4 in various lysates. Samples were incubated with Nectin 4 Polyclonal antibody (Product # PA5-110246) using a dilution of 1:2,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.