Immunohistochemistry analysis of Timeless in paraffin-embedded human gastric carcinoma tissue. Samples were heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, 20 min) and blocked in 5% BSA (30 min, room temperature), incubated with Timeless monoclonal antibody (Product # MA5-34880) at a dilution of 1:50 (30 min, room temperature), and followed by HRP conjugated compact polymer system, DAB, counterstained with hematoxylin (mounted with DPX).

Western blot analysis of Timeless in Positive control: Lane 1: HeLa cell lysate, Lane 2: 293T cell lysate, Lane 3: Daudi cell lysate. Samples were transferred to PVDF membrane, blocked with 5% BSA (1 hour), incubated with Timeless monoclonal antibody (Product # MA5-34880), at a dilution of 1:500, followed by Goat Anti-Rabbit IgG-HRP (1 hour) with a dilution of 1:5000.