Western blot analysis of AMT in LO2, SW620, 22Rv1, mouse pancreas, mouse brain, rat liver. Samples were incubated in polyclonal AMT antibody (Product # PA5-109385) using a dilution of 1:1000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10000.

Western blot analysis of AMT in extracts of various cell lines. Samples were incubated with AMT Polyclonal antibody (Product # PA5-109385) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.