Western blot analysis of THEM4 in extracts of various cell lines. Samples were incubated with THEM4 Polyclonal antibody (Product # PA5-110484) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
Western blot analysis of THEM4 in U-87MG, HeLa, mouse brain, mouse liver, mouse testis. Samples were incubated in polyclonal THEM4 antibody (Product # PA5-110484) using a dilution of 1:1000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10000.
Supplier Page from Thermo Fisher Scientific for THEM4 Antibody