Immunocytochemistry analysis of TPP2 in U-2OS cells. Samples were incubated in polyclonal TPP2 antibody (Product # PA5-110512) using a dilution of 1:100, followed by DAPI.

Western blot analysis of TPP2 in extracts of various cell lines. Samples were incubated with TPP2 Polyclonal antibody (Product # PA5-110512) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.