Western blot analysis of PI4KA in HeLa, NIH/3T3, mouse brain, rat brain. Samples were incubated in polyclonal PI4KA antibody (Product # PA5-109770) using a dilution of 1:1000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10000.

Western blot analysis of PI4KA in extracts of various cell lines. Samples were incubated with PI4KA Polyclonal antibody (Product # PA5-109770) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.