Knockdown of PRMT3 was achieved by transfecting A549 cells with specific validated siRNA (Silencer® select Product # s19870). Immunofluorescence analysis was performed on A549 cells (untransfected, panel a-d), transfected with PRMT3 specific siRNA (panel i-l) or non-specific scrambled siRNA (panels e-h). Cells were fixed, permeabilized, and labeled with Anti-PRMT3 Recombinant Rabbit Monoclonal Antibody (Product # 703744) at a 1:100 dilution, followed by Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a 1:2000 dilution. Nuclei (blue) were stained using ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962), and Rhodamine Phalloidin (Product # R415) at a 1:300 dilution was used for cytoskeletal F-actin (red) staining. Significant reduction of the signal was observed upon siRNA mediated knockdown (panel i-l) confirming the specificity of the antibody to PRMT3 (green). The images were captured at 60X magnification.