Western blot analysis of PCOLCE in extracts of U-251MG cells. Samples were incubated with PCOLCE Polyclonal antibody (Product # PA5-110084) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.