Western blot analysis of ATGL in A-549, HepG2, THP-1, A673. Samples were incubated in polyclonal ATGL antibody (Product # PA5-110189) using a dilution of 1:1000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10000.

Western blot analysis of ATGL in extracts of various cell lines. Samples were incubated with ATGL Polyclonal antibody (Product # PA5-110189) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.