Immunofluorescence analysis of POFUT2 in U2OS cells. Samples were incubated with POFUT2 Polyclonal antibody (Product # PA5-110192) using a dilution of 1:100. Blue: DAPI for nuclear staining.
Western blot analysis of POFUT2 in U-87MG, HepG2, mouse brain, mouse heart, rat brain, rat heart. Samples were incubated in polyclonal POFUT2 antibody (Product # PA5-110192) using a dilution of 1:1000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10000.
Western blot analysis of POFUT2 in extracts of various cell lines. Samples were incubated with POFUT2 Polyclonal antibody (Product # PA5-110192) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
Supplier Page from Thermo Fisher Scientific for POFUT2 Antibody