Western blot analysis of TOB2 in extracts of various cell lines. Samples were incubated with TOB2 Polyclonal antibody (Product # PA5-110504) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.
Immunohistochemistry analysis of TOB2 in paraffin-embedded human liver injury tissue. Samples were incubated in polyclonal TOB2 antibody (Product # PA5-110504) using a dilution of 1:100.
Immunohistochemistry analysis of TOB2 in paraffin-embedded human liver damage. Samples were incubated with TOB2 Polyclonal antibody (Product # PA5-110504) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Supplier Page from Thermo Fisher Scientific for TOB2 Antibody