Western blot analysis of MID2 in extracts of various cell lines. Samples were incubated with MID2 Polyclonal antibody (Product # PA5-109958) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 40s.
Western blot analysis of MID2 in BT-474, 293T, U-251MG, mouse lung. Samples were incubated in polyclonal MID2 antibody (Product # PA5-109958) using a dilution of 1:1000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10000.
Supplier Page from Thermo Fisher Scientific for MID2 Antibody