Immunocytochemistry analysis of NADE in MCF-7. Samples were incubated in polyclonal NADE antibody (Product # PA5-110029) using a dilution of 1:100.

Western blot analysis of NADE in extracts of various cell lines. Samples were incubated with NADE Polyclonal antibody (Product # PA5-110029) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.