Immunofluorescence analysis of GART in NIH-3T3 cells. Samples were incubated with GART Polyclonal antibody (Product # PA5-109755) using a dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
Western blot analysis of GART in extracts of various cell lines. Samples were incubated with GART Polyclonal antibody (Product # PA5-109755) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Western blot analysis of GART in U-87MG, HepG2, HeLa, K-562, mouse liver. Samples were incubated in polyclonal GART antibody (Product # PA5-109755) using a dilution of 1:1000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10000.
Immunofluorescence analysis of GART in U-2 OS cells. Samples were incubated with GART Polyclonal antibody (Product # PA5-109755) using a dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
Supplier Page from Thermo Fisher Scientific for GART Antibody