BALB/c mouse splenocytes were stained with CD45R (B220) Monoclonal Antibody, eFluor 450 (Product # 48-0452-82) and 0.03 µg of Recombinant Rabbit IgG Monoclonal Isotype Control, Alexa Fluor 488 (Product # 53-4616-82) (left) or 0.03 µg of CD124 Recombinant Rabbit Monoclonal Antibody, Alexa Fluor 488 (right). Viable cells were used for analysis, as determined by Fixable Viability Dye eFluor 780 (Product # 65-0865-18).